Rapid detection of Akabane virus by a novel reverse transcription loop-mediated isothermal amplification assay (RT-LAMP)

被引：12
|
作者：
Qiao, Jun [1 ]
Wang, Junwei [1 ]
Meng, Qingling [1 ,2 ]
Wang, Guochao [1 ]
Liu, Yucheng [1 ]
He, Zhihao [1 ]
Yang, Haibo [1 ]
Zhang, Zaichao [1 ]
Cai, Xuepeng [2 ]
Chen, Chuangfu [1 ]
机构：
[1] Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China
[2] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China
来源：
VIROLOGY JOURNAL | 2013年 / 10卷
基金：
中国国家自然科学基金;
关键词：
Rapid detection; Akabane virus; Reverse transcription loop-mediated isothermal amplification assay; LINKED-IMMUNOSORBENT-ASSAY; POLYMERASE-CHAIN-REACTION; VIRAL-ANTIGEN; ANTIBODIES; CATTLE; AINO; ENCEPHALOMYELITIS; JAPAN; ELISA;
D O I：
10.1186/1743-422X-10-288
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Background: Akabane disease, caused by Akabane virus, is an insect-transmitted disease of ruminants that is primarily characterized by fetal damage. Methods and results: In this study, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of Akabane virus was successfully developed. The primers were designed to target the highly conserved fragment of nucleoprotein from the Akabane virus. The results indicate that the assay is highly specific and sensitive with a detection limit of 5.0 TCID50(/)mL within a 60-min incubation time. A total of 126 abortive samples collected from Xinjiang province were detected by the established RT-LAMP. The results of RT-LAMP assay showed 96.8% agreement with the semi-nested RT-PCR. Conclusion: This study is to first to develop a rapid, sensitive, and accurate method for the detection of Akabane virus, which may be used to screen clinical samples in developing countries or regions.
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页数：5
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