Isolation and characterization of 23 microsatellite loci in Astragalus camptodontus (Leguminosae), a traditional medicinal plant in Yunnan province

被引：4
|
作者：
Zhang, Dequan [1 ]
Yang, Jing [2 ]
Jiang, Bei [1 ]
机构：
[1] Dali Univ, Coll Pharm & Chem, Dali 671000, Yunnan, Peoples R China
[2] Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Kunming 650201, Yunnan, Peoples R China
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2013年 / 50卷
基金：
中国国家自然科学基金;
关键词：
Astragalus camptodontus; Medicinal plant; Microsatellite; FIASCO; Genetic diversity; MARKERS;
D O I：
10.1016/j.bse.2013.06.011
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Astragalus camptodontus is a traditional medicinal plant in Yunnan province, and it is used as one of substitutes for the Chinese medicine Astragali radix, namely "Huangqi". Twenty-three microsatellite loci were developed from its nuclear genome using a fast isolation protocol by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Two wild populations collected from Northwest Yunnan were used to assess polymorphism of each locus. The number of alleles per locus ranged from 2 to 5 with a mean of 4.2. Values for Ho and HE ranged from 0.042 to 0.875 and from 0.254 to 0.793, with average of 0.403 and 0.616, respectively. These polymorphic loci will facilitate further studies on genetic diversity, population structure and gene flow of A. camptodontus and its allied species. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：448 / 451
页数：4
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