Long-term cryopreservation of sperm of Japanese eel

被引：0
|
作者：
Tanaka, S
Zhang, H
Horie, N
Yamada, Y
Okamura, A
Utoh, T
Mikawa, N
Oka, HP
Kurokura, H
机构：
[1] IRAGO Inst, Atsumi, Aichi 4413605, Japan
[2] Univ Tokyo, Bunkyo Ku, Tokyo 1138657, Japan
来源：
JOURNAL OF FISH BIOLOGY | 2002年 / 60卷 / 01期
关键词：
Japanese eel; sperm; cryopreservation; sodium bicarbonate; hatchability;
D O I：
10.1006/jfbi.2001.1819
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
After cryopreservation in a diluent (D#5-D), containing 76.2 mM NaHCO3, 137.0 mm NaCl, 1.4% soya lecithin and dimethyl sulphoxide, for 30-180 days, Japanese eel Anguilla japonica sperm was 61.4-70.6% motile compared with fresh sperm. Cryopreserved sperm was used for fertilizing three batches of eggs and hatchability ranged from 20.7 to 69.1%. These results indicate that D#5-D is an useful diluent for sperm cryopreservation in Japanese eel. (C) 2002 The Fisheries Society of the British Isles.
引用
收藏
页码：139 / 146
页数：8
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