Long-term cryopreservation of sperm of Japanese eel

被引：0

作者：

Tanaka, S

Zhang, H

Horie, N

Yamada, Y

Okamura, A

Utoh, T

Mikawa, N

Oka, HP

Kurokura, H

机构：

[1] IRAGO Inst, Atsumi, Aichi 4413605, Japan

[2] Univ Tokyo, Bunkyo Ku, Tokyo 1138657, Japan

来源：

JOURNAL OF FISH BIOLOGY | 2002年 / 60卷 / 01期

关键词：

Japanese eel; sperm; cryopreservation; sodium bicarbonate; hatchability;

D O I：

10.1006/jfbi.2001.1819

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

After cryopreservation in a diluent (D#5-D), containing 76.2 mM NaHCO3, 137.0 mm NaCl, 1.4% soya lecithin and dimethyl sulphoxide, for 30-180 days, Japanese eel Anguilla japonica sperm was 61.4-70.6% motile compared with fresh sperm. Cryopreserved sperm was used for fertilizing three batches of eggs and hatchability ranged from 20.7 to 69.1%. These results indicate that D#5-D is an useful diluent for sperm cryopreservation in Japanese eel. (C) 2002 The Fisheries Society of the British Isles.

引用

页码：139 / 146

页数：8

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