Biochemical and molecular characterizations of multidrug resistance bacteria isolated from Saudi Arabian hospital

One of the well-recognized problems nowadays is the epidemic of the resistance of microorganisms to antimicrobial agents. So, this descriptive, cross-sectional, hospital-based study starts to screen the patient's samples to search for most antibiotic-resistant bacteria. A total of 120 isolates from outpatients and inpatients were used to identify the most MDR bacteria. A 120 samples was collected at King Abdullah Medical Complex. VITEK? 2 (BioMerieux, France) and MicroScan Microbiology system (Siemens, Germany) were used in isolates identification and antimicrobial susceptibility test. So, the highest prevalence of bacterial isolates was identified as Klebsiella pneumoniae 26 (21.66%), Escherichia coli 23 (19.16%), Pseudomonas aeruginosa 16 (13.33%) and Staphylococcus aureus 15 (12.5%). The most resistant sample was selected based on the number of antibiotics it was susceptible to. K. pneumoniae , found to be resistant to 16 antibiotics. Furthermore, the isolate is identified as OXA-48-Positive Carbapenem-Resistant K. pneumoniae.