Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene

被引：53
|
作者：
Kim, CK
Chung, JD
Park, SH
Burrell, AM
Kamo, KK
Byrne, DH [1 ]
机构：
[1] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA
[2] Sangju Natl Univ, Dept Hort, Sangju 742711, South Korea
[3] Kyungpook Natl Univ, Dept Hort, Taegu 702701, South Korea
[4] ARS, USDA, Florist & Nursery Plants Res Unit, Beltsville, MD 20705 USA
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2004年 / 78卷 / 02期
关键词：
genetic engineering; marker gene; rose; transgenic plants; vir gene;
D O I：
10.1023/B:TICU.0000022529.16697.90
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.
引用
收藏
页码：107 / 111
页数：5
相关论文
共 50 条
  • [31] Agrobacterium tumefaciens-mediated transformation of Robinia pseudoacacia
    T. Igasaki
    T. Mohri
    H. Ichikawa
    K. Shinohara
    Plant Cell Reports, 2000, 19 : 448 - 453
  • [32] Agrobacterium tumefaciens-mediated transformation of filamentous fungi
    Marcel J.A. de Groot
    Paul Bundock
    Paul J.J Hooykaas
    Alice G.M. Beijersbergen
    Nature Biotechnology, 1998, 16 : 839 - 842
  • [33] Agrobacterium tumefaciens-mediated transformation of Atropa belladonna
    Song, Guo-qing
    Walworth, Aaron
    PLANT CELL TISSUE AND ORGAN CULTURE, 2013, 115 (01) : 107 - 113
  • [34] Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid
    Liau, CH
    You, SJ
    Prasad, V
    Hsiao, HH
    Lu, JC
    Yang, NS
    Chan, MT
    PLANT CELL REPORTS, 2003, 21 (10) : 993 - 998
  • [35] Agrobacterium tumefaciens-mediated transformation of Rhipsalidopsis gaertneri
    E. A. Al-Ramamneh
    S. Sriskandarajah
    M. Serek
    Plant Cell Reports, 2006, 25 : 1219 - 1225
  • [36] Agrobacterium tumefaciens-Mediated Transformation of Recalcitrant Crops
    van Wordragen, Monique F.
    Dons, Hans J. M.
    PLANT MOLECULAR BIOLOGY REPORTER, 1992, 10 (01) : 12 - 36
  • [37] Agrobacterium tumefaciens-mediated transformation of CryⅠA(b) gene to Trichoderma harzianum
    GAO Xingxi & YANG Qian Department of Life Science and Engineering
    ChineseScienceBulletin, 2004, (23) : 2491 - 2494
  • [38] Agrobacterium tumefaciens-mediated GUS gene transformation of Robinia pseudoacacia 'Idaho'
    Sun Hai-jun Li Min Chen Shou-yi He Si-jie Wang Hua-fang College of Biological Sciences and Biotechnology
    Forest Ecosystems, 2006, (04) : 63 - 68
  • [39] The green fluorescent protein as an efficient selection marker for Agrobacterium tumefaciens-mediated transformation in Hevea brasiliensis (Müll. Arg)
    J. Leclercq
    L. Lardet
    F. Martin
    T. Chapuset
    G. Oliver
    P. Montoro
    Plant Cell Reports, 2010, 29 : 513 - 522
  • [40] Agrobacterium tumefaciens -mediated transformation of modern rose ( Rosa hybrida ) using leaf-derived embryogenic callus
    Liu, Guoqin
    Yuan, Yuan
    Jiang, Hui
    Bao, Ying
    Ning, Guogui
    Zhao, Liangjun
    Zhou, Xiaofeng
    Zhou, Hougao
    Gao, Junping
    Ma, Nan
    HORTICULTURAL PLANT JOURNAL, 2021, 7 (04) : 359 - 366
12345