Facile immobilization of nitrile hydratase in SBA-15 via a biomimetic coating

被引：8
|
作者：
Chen, Yan [1 ]
Jiang, Yanjun [2 ]
Gao, Junkai [1 ]
Wu, Wenfeng [1 ]
Dong, Liangxiong [1 ]
Yang, Zejun [1 ]
机构：
[1] Zhejiang Ocean Univ, Sch Port & Transportat Engn, Zhoushan 316022, Peoples R China
[2] Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China
来源：
JOURNAL OF POROUS MATERIALS | 2017年 / 24卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Polyopamine; Biomimetic coating; Facile immobilization; Nitrile hydratase; MESOPOROUS SILICA; HORSERADISH-PEROXIDASE; EFFICIENT REMOVAL; LIPASE; ENZYME; NANOPARTICLES;
D O I：
10.1007/s10934-016-0317-6
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Dopamine functionalized mesoporous molecular sieve SBA-15 (Dop-SBA-15), synthesized by a simple and cost-effective method, was developed as a matrix to immobilize NHase (NHase@Dop-SBA-15). Various characterization techniques such as SEM, TEM, BET and FT-IR were carried out to investigate the properties of Dop-SBA-15 and NHase@Dop-SBA-15. It was shown that the Dop-SBA-15 had highly ordered mesopores and large surface area of 447 m(2)/g. The activity, thermal stability, pH stability, storage stability, and reusability of NHase@Dop-SBA-15 were studied in detail. It was observed that the performance of NHase@Dop-SBA-15 was improved significantly compared to that of the free NHase and NHase immobilized in unfunctionalized SBA-15. This study provided insight into the preparation of Dop-SBA-15 and its application for NHase immobilization.
引用
收藏
页码：787 / 793
页数：7
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