Single nucleotide polymorphisms of Kit gene in Chinese indigenous horses

被引:1
|
作者
Han, Haoyuan [1 ]
Mao, Chunchun [1 ]
Chen, Ningbo [1 ]
Lan, Xianyong [1 ]
Chen, Hong [1 ]
Lei, Chuzhao [1 ]
Dang, Ruihua [1 ]
机构
[1] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Chinese horses; Kit; SNPs; white coat color; COAT COLOR PHENOTYPES; STEM-CELL FACTOR; TYROSINE KINASE; MELANOBLASTS; ACTIVATION; EXPRESSION; MIGRATION; MUTATIONS; PATTERN; LIGAND;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Kit gene is a genetic determinant of horse white coat color which has been a highly valued trait in horses for at least 2,000 years. Single nucleotide polymorphisms (SNPs) in Kit are of importance due to their strong associations with melanoblast survival during embryonic development. In this study, a mutation analysis of all 21 Kit exons in 14 Chinese domestic horse breeds revealed six SNPs (g.91214T>G, g.143245T>G, g.164297C>T, g.170189C>T, g.171356C>G, and g.171471G>A), which located in 5'-UTR region, intron 6, exon 15, exon 20, intron 20, and exon 21 of the equine Kit gene, respectively. Subsequently, these six SNPs loci were genotyped in 632 Chinese horses by PCR-RFLP or direct sequencing. The six SNPs together defined 18 haplotypes, demonstrating abundant haplotype diversities in Chinese horses. All the mutant alleles and haplotypes were shared among different breeds. But fewer mutations were detected in horses from China than that from abroad, indicating that Chinese horses belong to a more ancient genetic pool. This study will provide fundamental genetic information for evaluating the genetic diversity of Kit gene in Chinese indigenous horse breeds.
引用
收藏
页码:81 / 89
页数:9
相关论文
共 50 条
  • [21] Single nucleotide polymorphisms of leptin gene in five Ethiopian indigenous cattle breeds and the Korean Hanwoo breed
    Ayele Mandefro
    Tesfaye Sisay
    Kwan-Suk Kim
    Zewdu Edea
    Rocktotpal Konwarh
    Hailu Dadi
    Tropical Animal Health and Production, 2021, 53
  • [22] Single nucleotide polymorphisms of leptin gene in five Ethiopian indigenous cattle breeds and the Korean Hanwoo breed
    Mandefro, Ayele
    Sisay, Tesfaye
    Kim, Kwan-Suk
    Edea, Zewdu
    Konwarh, Rocktotpal
    Dadi, Hailu
    TROPICAL ANIMAL HEALTH AND PRODUCTION, 2021, 53 (02)
  • [23] Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit
    Zhao, CX
    Xu, GW
    Shi, XZ
    Ma, JM
    Zhang, Y
    Lv, S
    Yang, Q
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2003, 795 (01): : 55 - 60
  • [24] Correlation between single nucleotide polymorphisms in a calprotectin subunit gene and risk of periodontitis in a Chinese population
    Li, QiYan
    Meng, HuanXin
    Zhang, Li
    Xu, Li
    Chen, ZhiBin
    Shi, Dong
    Feng, XiangHui
    Zhu, XiaoLing
    Zhao, HongShan
    Cao, CaiFang
    ANNALS OF HUMAN GENETICS, 2007, 71 : 312 - 324
  • [25] Two single nucleotide polymorphisms in TSLP gene are associated with asthma susceptibility in Chinese Han population
    Liu, Wen
    Xu, Li-Sheng
    Liu, Qi-Ji
    Dong, Fang-Zheng
    Qiu, Rong-Fang
    Wen, Ming-Chun
    Han, Yu-Ling
    Tang, Ning-Bo
    Kang, Li-Jun
    Wu, Jin-Xiang
    Liu, Fen
    Zhao, Ji-Ping
    Yang, Meng-Meng
    Wang, Jun-Fei
    Ding, Ming-Jie
    Sun, Yue-Mei
    Fei, Wen-Jian
    Dong, Liang
    EXPERIMENTAL LUNG RESEARCH, 2012, 38 (08) : 375 - 382
  • [26] Single nucleotide polymorphisms of the SCN5A gene in Han Chinese and their relation with Brugada syndrome
    Chen, JZ
    Xie, XD
    Wang, XX
    Tao, M
    Shang, YP
    Guo, XG
    CHINESE MEDICAL JOURNAL, 2004, 117 (05) : 652 - 656
  • [27] Association between schizophrenia and single nucleotide polymorphisms in lipoprotein lipase gene in a Han Chinese population
    Xie, Cui
    Wang, Zeng Chan
    Liu, Xiao Feng
    Wang, Li
    Yang, Mao Sheng
    PSYCHIATRIC GENETICS, 2011, 21 (06) : 307 - 314
  • [28] Association of single nucleotide polymorphisms of IL-6 gene with frailty in the Chinese Bai nationality
    Li, Xueqiong
    Yang, Yan
    Meng, Qi
    Shen, Yun
    MEDICINE, 2023, 102 (36) : E35010
  • [29] Clinical significance of single nucleotide polymorphisms of adiponectin gene in Chinese benign prostatic hyperplasia patients
    Gu, Xin
    Xu, Ding
    Huang, Tao
    Duan, Liujian
    Jiao, Yang
    Qi, Jun
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2017, 10 (02): : 2169 - 2174
  • [30] Single nucleotide polymorphisms of the SCN5A gene in Han Chinese and their relation with Brugada syndrome
    陈君柱
    谢旭东
    王兴祥
    陶明
    尚云鹏
    郭晓钢
    ChineseMedicalJournal, 2004, (05)