Lab on a chip automates in vitro cell culturing

被引：15

作者：

Perozziello, Gerardo ^{[1
]}

Mollenbach, Jacob ^{[2
]}

Laursen, Steen ^{[2
]}

Di Fabrizio, Enzo ^{[3
]}

Gernaey, Krist ^{[4
]}

Kruhne, Ulrich ^{[4
]}

机构：

[1] Magna Graecia Univ Catanzaro, Dept Expt & Clin Med, Lab BioNEM Bio Nano Engn & Technol Med, I-88100 Catanzaro, Italy

[2] Smart Biosyst ApS, DK-2400 Copenhagen NW, Denmark

[3] Italian Inst Technol, Nanostruct Dept, I-16163 Genoa, Italy

[4] Tech Univ Denmark, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark

来源：

MICROELECTRONIC ENGINEERING | 2012年 / 98卷

关键词：

Lab on a chip; Automated in vitro fertilization; Single cell cultivation; T and pH control; Rapid prototyping;

D O I：

10.1016/j.mee.2012.07.027

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A novel in vitro fertilization system is presented based on an incubation chamber and a microfluidic device which serves as advanced microfluidic cultivation chamber. The flow is controlled by hydrostatic height differences and evaporation is avoided with help of mineral oil. Six patient compartments allow six simultaneous temperature and pH controlled cultivations with 12 embryos with continuous logging of the monitoring data. Two media can be controlled with help of opening or closing of openings at the microfluidic disposable devices. The flow rates through the single cell compartments can be controlled up to 20 mu l/h. A common pH electrode is supplied by 14 mu l sample, which is expanded with help of DI water. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：655 / 658

页数：4

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