Alcanivorax sediminis sp. nov., isolated from deep-sea sediment of the Pacific Ocean

A taxonomic study was carried out on strain PA15-N-34(T), which was isolated from deep-sea sediment of Pacific Ocean. The bacterium was Gram-stain-positive, oxidase- and catalase-positive and rod-shaped. Growth was observed at salinity of 0-15.0% NaCl and at temperatures of 10-45 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PA15-N-34(T) belonged to the genus Alcanivorax, with the highest sequence similarity to Alcanivorax profundi MTEO17(T) (97.7 %), followed by Alcanivorax nanhaiticus 19 m-6(T) (97.3 %) and 12 other species of the genus Alcanivorax (93.4 %-97.0%). The average nucleotide identity and DNA-DNA hybridization values between strain PA15-N-34(T) and type strains of the genus Alcanivorax were 71.46-81.78% and 18.7-25.2%, respectively. The principal fatty acids (>10%) were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c; 31.2%), C-16:0 (25.0%) and summed feature 3 (14.6%). The DNA G+C content was 57.15 mol%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, four unidentified aminolipids and three unidentified lipids. The novel strain can be differentiated from its closest type strain by a negative test for urease and the presence of diphosphatidylglycerol and aminolipid. The combined genotypic and phenotypic data show that strain PA15-N-34(T) represents a novel species within the genus Alcanivorax, for which the name Alcanivorax sediminis sp. nov. is proposed, with the type strain PA15-N-34(T) (=MCCC 1A14738(T)=KCTC 72163(T)).