Metabolic vasodilation in the human forearm is preserved in hypercholesterolemia despite impairment of endothelium-dependent and independent vasodilation

Objective: Hypercholesterolemia has been shown to impair endothelium-mediated, nitric oxide (NO)-dependent responses to acetylcholine (ACh), serotonin, substance P and flow-mediated dilation. We have recently shown that NO contributes to metabolic vasodilation in the human forearm. We sought to determine whether metabolic vasodilation is impaired in healthy subjects with hypercholesterolemia. Methods: We compared the forearm blood flow (FBF) responses to isotonic exercise, ACh and the endothelium-independent vasodilator sodium nitroprusside in young, otherwise healthy volunteers with hypercholesterolemia and controls before and after the NO inhibitor N-G-monomethyl-L-arginine (L-NMMA). FBF was measured using venous occlusion plethysmography, Hy percholesterolemic (n=20) and control(n=20) subjects were age- and gender-matched. Results: Total cholesterol(6.9+/-0.3 vs. 4.6+/-0.1 mmol/l, P<0.0001), low density lipoprotein (4.9+/-0.4 vs. 2.7+/-0.1 mmol/l, P<0.001) and triglyceride (1.3+/-0.2 vs. 0.8+/-0.1 mmol/l, P=0.005) levels were higher in the hypercholesterolemic group. Basal FBF and resistance were similar in the two groups. Hypercholesterolemia impaired the peak FBF response to ACh (11.1+/-1.9 vs. 17.6+/-2.2 ml/100 ml/min, P=0.03), and reduced the peak response to sodium nitroprusside (6.0=/-0.4 vs. 8.1+/-0.6 ml/100 ml/min, P<0.01). However, hypercholesterolemia did not affect peak hyperemic FBF (13.1+/-1.0 vs. 13.2+/-1.0 ml/100 ml/min, P=1.0) or the FBF volume repayment during the 1 or 5 min after exercise. Resting FBF was reduced by L-NMMA to a similar degree (by 33% vs. 40%, P=0.17) in both groups. Although L-NMMA reduced peak hyperemic FBF (by 16% vs. 17%, P=0.93) and the volume repaid after exercise in both groups, there were no differences between the two groups. Conclusions: Exercise-induced metabolic vasodilation is in part dependent on NO release. Hypercholesterolemia impairs NO-mediated vasodilation, but is not associated with a reduction in exercise-induced hyperemia. This may indicate that multiple compensatory mechanisms are operative in skeletal muscle metabolic vasodilation. (C) 1999 Elsevier Science B.V. All rights reserved.