Comparison of Ammonia-Oxidizing Bacterial Community Structure in Membrane-Assisted Bioreactors Using PCR-DGGE and FISH

被引：6
|
作者：
Ziembinska, A. [1 ]
Ciesielski, S. [2 ]
Gnida, A. [1 ]
Zabczynski, S. [1 ]
Surmacz-Gorska, J. [1 ]
Miksch, K. [1 ]
机构：
[1] Silesian Tech Univ, Environm Biotechnol Dept, PL-44100 Gliwice, Poland
[2] Univ Warmia & Mazury, Dept Environm Biotechnol, PL-10719 Olsztyn, Poland
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2012年 / 22卷 / 08期
关键词：
Activated sludge; ammonia-oxidizing bacteria; PCR-DGGE; FISH; MBR; 16S rRNA gene; GRADIENT GEL-ELECTROPHORESIS; SITU HYBRIDIZATION FISH; ACTIVATED-SLUDGE; LANDFILL LEACHATE; WASTE-WATER; MOLECULAR ANALYSIS; DIVERSITY; MBR;
D O I：
10.4014/jmb.1201.01014
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The ammonia-oxidizing bacterial (AOB) communities in three membrane bioreactors (MBRs) were monitored for 2 months after an acclimation period in order to investigate the influence of sludge age and medium type on AOB changeability and its connection with nitrification effectiveness. One MBR with a sludge age of 4 days was fed with a synthetic medium, whereas the other two with sludge ages of 8 and 32 days were fed with landfill leachate. The research revealed that landfill leachate can be effectively treated in an MBR with a higher sludge age for longer periods of time and that this improvement in performance was correlated with an increase in AOB biodiversity. Interestingly, the medium type has a stronger influence on AOB biocenosis formation than the sludge age.
引用
收藏
页码：1035 / 1043
页数：9
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