Identification and validation of microsatellite markers linked to the Rpp5 gene conferring resistance to Asian soybean rust

被引：8
|
作者：
Silva Morceli, Thaiza Galhardo [1 ]
Uneda Trevisoli, Sandra Helena [2 ]
Morceli, Antonio Ayrton, Jr. [1 ]
de Souza Kiihl, Romeu Afonso [2 ]
Calvo, Eberson Sanches [2 ]
Di Mauro, Antonio Orlando [1 ]
Garcia, Alexandre [3 ]
机构：
[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Prod Vegetal, BR-14884900 Jaboticabal, SP, Brazil
[2] Fac Tecnol Jaboticabal, BR-14884900 Jaboticabal, SP, Brazil
[3] Trop Melhoramento & Genet Ltda, BR-86183600 Cambe, PR, Brazil
来源：
PESQUISA AGROPECUARIA BRASILEIRA | 2008年 / 43卷 / 11期
关键词：
Glycine max; Phakopsora pachyrhizi; marker assisted selection; SSR;
D O I：
10.1590/S0100-204X2008001100011
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The main objective of this work was to identify new microsatellite markers, linked to the Rpp5 resistance gene to Asian soybean rust, and to validate previously mapped markers for use in marker-assisted selection (MAS) programs. To this end, a F-2 population with 100 individuals, derived from crossing between PI 200526 and cultivar Coodetec 208, susceptible to rust, was artificially infected and evaluated for its reaction of resistance to rust. Microsatellite markers were tested on parents and in the two contrasting bulks to identifying linked markers. Two new markers, potentially associated with resistance, were tested in individual plants, and they were found to be linked to gene Rpp5 and to be present in the N linkage group of soybean. The selection efficiencies were determined for all markers linked to gene Rpp5, and the combination of the markers Sat_275+Sat_280 was 100%.
引用
收藏
页码：1525 / 1531
页数：7
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