A multiplex PCR method for simultaneous species identification and strain typification of Oenococcus oeni

被引：4
|
作者：
Araque, Maria Isabel [1 ]
Bordons, Albert [1 ]
Reguant, Cristina [1 ]
机构：
[1] Univ Rovira & Virgili, Dept Bioquim & Biotechnol, Tarragona 43007, Catalonia, Spain
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2009年 / 25卷 / 01期
关键词：
Oenococcus oeni; Malolactic fermentation; Wine; Multiplex PCR; Identification; Strain typification; LACTIC-ACID BACTERIA; MALOLACTIC FERMENTATION; LEUCONOSTOC-OENOS; RED WINE; DNA;
D O I：
10.1007/s11274-008-9854-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Selected starter cultures of Oenococcus oeni are widely used to initiate malolactic fermentation (MLF) in wine. Nevertheless, the inoculated culture does not always develop as expected and undesired strains can grow causing wine spoilage. Therefore, methods that can reliably differentiate O. oeni strains are essential to monitor the population dynamics of MLF. This work presents a new multiplex PCR method that allows the simultaneous species identification and strain typification of O. oeni, based on the combined use of species-specific PCR primers and a Random Polymorphic DNA (RAPD)-PCR primer. This method represents an useful tool for the control of wine MLF.
引用
收藏
页码：15 / 18
页数：4
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