Identification of the free phenolic profile of Adlay bran by UPLC-QTOF-MS/MS and inhibitory mechanisms of phenolic acids against xanthine oxidase

被引：63
|
作者：
Lin, Lianzhu [1 ,3 ]
Yang, Qingyun [1 ,3 ]
Zhao, Kun [1 ,3 ]
Zhao, Mouming [1 ,2 ,3 ]
机构：
[1] South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China
[2] Beijing Technol & Business Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China
[3] Guangdong Food Green Proc & Nutr Regulat Technol, Guangzhou 510640, Guangdong, Peoples R China
来源：
FOOD CHEMISTRY | 2018年 / 253卷
基金：
中国国家自然科学基金;
关键词：
Adlay bran; UPLC-QTOF-MS/MS; Phenolic acids; Xanthine oxidase inhibitory mechanism; Structure-activity relationship; Interaction; MA-YUEN STAPF; ANTIOXIDANT ACTIVITY; DIETARY FLAVONOIDS; OXIDATIVE STRESS; EXTRACTS; JOBI; L; PHYTOCHEMICALS; HYPERURICEMIA; GALANGIN;
D O I：
10.1016/j.foodchem.2018.01.139
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Adlay bran free phenolic extract has been previously demonstrated to possess potent xanthine oxidase (XOD) inhibitory activity. The aims of this study were to characterize the free phenolic profile of adlay bran and investigate the structure-activity relationship, underlying mechanism and interaction of phenolic acids as XOD inhibitors. A total of twenty phenolics including ten phenolic acids, two coumarins, two phenolic aldedhyes and six flavonoids were identified in a phenolic compound-guided separation by UPLC-QTOF-MS/MS. Adlay bran free phenolic extract possessed strong XOD inhibitory activity related to hydroxycinnamic acids with methoxyl groups. The hydrogen bonding and hydrophobic interactions were the main forces in the binding of adlay phenolics to XOD. Sinapic acid, identified in adlay bran for the first time, possessed strong XOD inhibitory activity in a mixed non-competitive manner, and synergistic effects with other adlay phenolic acids at low concentrations, and would be a promising agent for preventing and treating hyperuricemia.
引用
收藏
页码：108 / 118
页数：11
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