The morphological correlate of deafness is the loss of hair cells with subsequent degeneration of spiral ganglion neurons (SGN). Neurotrophic factors have a neuroprotective effect, and especially brain-derived neurotrophic factor (BDNF) has been demonstrated to protect SGN in vitro and after ototoxic trauma in vivo. Erythropoietin (EPO) attenuates hair cell loss in rat cochlea explants that were treated with gentamycin. Recently, it has also been shown that EPO reduces the apoptose rate in hippocampal neurons. Therefore, the aim of the study was to examine the effects of EPO on SGN in vitro. Spiral ganglion cells were isolated from neonatal rats and cultured for 48 h in serum-free medium supplemented with EPO and/or BDNF. Results showed that survival rates of SGN were not significantly improved when cultivated with EPO alone. Also, EPO did not further increase BDNF-induced survival of SGN. However, significant elongation of neurites was determined when SGN were cultivated with EPO alone. Even though a less than additive effect was observed, combined treatment with BDNF and EPO led to a significant elongation of neurites when compared to individual treatment with BDNF or EPO. It can be concluded that EPO induces neurite outgrowth rather than promoting survival. Thus, EPO presents as an interesting candidate to enhance and modulate the regenerative effect of BDNF on SGN. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
机构:
Hanyang Univ, Dept Psychiat, Coll Med, Seoul 133791, South Korea
Hanyang Univ, Inst Mental Hlth, Seoul 133791, South KoreaHanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South Korea
Oh, Dong Hoon
Lee, In Young
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Hanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South KoreaHanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South Korea
Lee, In Young
Choi, Miyeon
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Hanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South KoreaHanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South Korea
Choi, Miyeon
Kim, Seok Hyeon
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Hanyang Univ, Dept Psychiat, Coll Med, Seoul 133791, South Korea
Hanyang Univ, Inst Mental Hlth, Seoul 133791, South KoreaHanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South Korea
Kim, Seok Hyeon
Son, Hyeon
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Hanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South KoreaHanyang Univ, Dept Biochem & Mol Biol, Coll Med, Seoul 133791, South Korea
Son, Hyeon
KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY,
2012,
16
(04):
: 281
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285
机构:
Univ Calif San Diego, Sch Med, Dept Surg Otolaryngol, La Jolla, CA 92093 USAUniv Calif San Diego, Sch Med, Dept Surg Otolaryngol, La Jolla, CA 92093 USA
Evans, Amaretta R.
Euteneuer, Sara
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机构:Univ Calif San Diego, Sch Med, Dept Surg Otolaryngol, La Jolla, CA 92093 USA
Euteneuer, Sara
Chavez, Eduardo
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机构:Univ Calif San Diego, Sch Med, Dept Surg Otolaryngol, La Jolla, CA 92093 USA
Chavez, Eduardo
Mullen, Lina M.
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Mullen, Lina M.
Hui, Elliot E.
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机构:Univ Calif San Diego, Sch Med, Dept Surg Otolaryngol, La Jolla, CA 92093 USA
Hui, Elliot E.
Bhatia, Sangeeta N.
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Bhatia, Sangeeta N.
Ryan, Allen F.
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机构:Univ Calif San Diego, Sch Med, Dept Surg Otolaryngol, La Jolla, CA 92093 USA
机构:
Toho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, JapanToho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, Japan
Iwasaki, Y
Ikeda, K
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Toho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, JapanToho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, Japan
Ikeda, K
Ichikawa, Y
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Toho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, JapanToho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, Japan
Ichikawa, Y
Igarashi, O
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Toho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, JapanToho Univ, Ohashi Hosp, Dept Internal Med 4, Meguro Ku, Tokyo 1538515, Japan