Fullerene-oxygen action on biological tissues: Numerical modeling.

A kinetic model of the photodynamic fullerene-oxygen action on biological tissues is developed. The efficiency of generation of singlet oxygen is studied in relation to the intensity of exciting radiation and the concentrations of fullerene and oxygen. The spectral efficiencies of singlet oxygen generation upon irradiation of a biological tissue by a lamp and different lasers are studied and compared with each other. It is shown that the singlet oxygen generation effectiveness increases with exciting wavelength decreasing. The spatial distributions of singlet oxygen in allantoic fluid is studied. The oxidation of lipids of external membranes is assumed to be the basic mechanism of cell damage. The characteristic time of this oxidation is calculated. The sensitivity of Semliki Forest Virus to photodynamic action is estimated. It's value can be used in calculations of the dose curves for this virus culture in other illumination conditions and for other fullerene and oxygene concentrations. The effectiveness of singlet oxygen generation using pulsed excitation is compared with the one using CW.