Regulation of uncoupling protein 2 expression by long-chain fatty acids and hormones in bovine mammary epithelial cells

被引：30
|
作者：
Yonezawa, Tomo [1 ]
Sanosaka, Masato [1 ]
Haga, Satoshi [1 ]
Kobayashi, Yosuke [1 ]
Katoh, Kazuo [1 ]
Obara, Yoshiaki [1 ]
机构：
[1] Tohoku Univ, Dept Anim Physiol, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 375卷 / 02期
关键词：
mammary epithelial cells; uncoupling proten 2; long-chain fatty acids; insulin; dexamethazone;
D O I：
10.1016/j.bbrc.2008.08.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Although mammary epithelial cells are known to synthesize and accumulate triacylglycerol (TAG) in order to produce milk lipid in the cytosol, lipid and energy metabolism is still not fully understood. In this study, we assessed the effects of long-chain fatty acid (LCFA) on the accumulation Of cytosolic TAG and uncoupling protein (UCP) 2 in cloned bovine mammary epithelial cells (bMEC). LCFAs significantly raised the expression of UCP2 mRNA and the accumulation of TAG. We observed the rapid elevation in UCP2 shown at 6 h after LCFA treatment. Insulin (5-50 ng/ml) or dexamethasone (500 nM) significantly suppressed the expression of UCP2 mRNA. These results suggest that UCP2 play an important role of lipid and energy metabolism in mammary epithelial cells. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：280 / 285
页数：6
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