Study of ethidium bromide interaction peculiarities with DNA

被引：17
|
作者：
Vardevanyan, PO
Antonyan, AP
Manukyan, GA
Karapetyan, AT
机构：
[1] Yerevan State Univ, Fac Biol, Dept Biophys, Yerevan 375025, Armenia
[2] Yerevan State Univ Architecture & Construct, Dept Phys, Yerevan 375009, Armenia
来源：
EXPERIMENTAL AND MOLECULAR MEDICINE | 2001年 / 33卷 / 04期
关键词：
DNA; ethidium bromide; mode of binding; bell-shape curve; melting curve;
D O I：
10.1038/emm.2001.34
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M less than or equal to mu(Na+) less than or equal to 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex - (DeltaT) becomes equal to that of DNA itself (DeltaT(0)). It has been shown that the values of rb at which deltaDeltaT=DeltaT-DeltaT(0)=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA.
引用
收藏
页码：205 / 208
页数：4
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