Assembly of a Modular Fluorimeter and Associated Software: Using LabVIEW in an Advanced Undergraduate Analytical Chemistry Laboratory

被引:20
|
作者
Algar, W. Russ [1 ]
Massey, Melissa [1 ]
Krull, Ulrich J. [1 ]
机构
[1] Univ Toronto, Chem Sensors Grp, Dept Chem & Phys Sci, Mississauga, ON L5L 1C6, Canada
关键词
DATA-ACQUISITION; FLUORESCENCE; COMPLEXES; IODIDE; SYSTEM;
D O I
10.1021/ed086p68
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We have described a laboratory activity for an upper-level undergraduate course in instrumental analysis that teaches the fundamentals of LabVIEW and interfacing through construction of a fluorimeter from modular components. Students use a DAQ module and LabVIEW to interface a desktop computer with a blue LED light source and photodiode detector. In addition to measuring a calibration curve for fluorescein, students have investigated the Stern-Volmer quenching of fluorescein by iodide and the pH dependence of fluorescein fluorescence.
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页码:68 / 71
页数:4
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