Quantitative assay of element mass inventories in single cell biological systems with micro-PIXE

被引:7
|
作者
Ogrinc, Nina [1 ,5 ]
Pelicon, Primoz [1 ]
Vavpetic, Primoz [1 ]
Kelemen, Mitja [1 ]
Grlj, Natasa [1 ]
Jeromel, Luka [1 ]
Tomic, Sergej [2 ]
Colic, Miodrag [2 ,3 ]
Beran, Alfred [4 ]
机构
[1] Jozef Stefan Inst, SI-1000 Ljubljana, Slovenia
[2] Univ Def, Fac Med, Mil Med Acad, Belgrade, Serbia
[3] Univ Nis, Med Fac, Nish 18000, Serbia
[4] Ist Nazl Oceanog & Geofis Sperimentale, Dipartimento Oceanog Biol, I-34151 Trieste, Italy
[5] LOTRIC Metrol, SI-4227 Selca, Slovenia
来源
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 2013年 / 306卷
关键词
Gold nanoparticles; Tinntinopsis radix; Zooplankton; Lorica; Element inventory; Dendritic cells; Particle induced X-ray emission-PIXE; Elastic backscattering spectroscopy-EBS; Scanning transmission ion microscopy-STM; Focused proton beam; Trace elements; Titanium dioxide particles; NANOPARTICLES;
D O I
10.1016/j.nimb.2012.12.060
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Elemental concentrations in micro-PIXE (Particle Induced X-ray Emission) maps of elements in biological tissue slices have been determined using auxiliary information on the sample matrix composition from EBS (Elastic Backscattering Spectroscopy) and STIM (Scanning Transmission Ion Microscopy). The thin sample approximation may be used for evaluating micro-PIXE data in cases, where X-ray absorption in the sample can be neglected and the mass of elements in a selected area can be estimated. The resulting sensitivity amounts to an impressive 10(-12) g of the selected elements. Two cases are presented as examples. In the first, we determined the total mass of gold nanoparticles internalized by human monocyte-derived dendritic cells (MDDC). In the second, an inventory of the mass of elements in the micro-particulate material adsorbed at the wall of the lorica of the microzooplankton species Tintinnopsis radix has been created. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:121 / 124
页数:4
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