Diversity of Y region at HML locus in a Saccharomyces cerevisiae strain isolated from a Sardinian wine

被引：4
|
作者：
Pirino, G [1 ]
Zara, S [1 ]
Pinna, G [1 ]
Farris, GA [1 ]
Budroni, M [1 ]
机构：
[1] Univ Sassari, DiSAABA, Sez Microbiol Gen & Applicata, I-07100 Sassari, Italy
来源：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2004年 / 85卷 / 01期
关键词：
flor strain; mating type switching; Saccharomyces cerevisiae; semi-homothallic life cycle;
D O I：
10.1023/B:ANTO.0000020269.80900.45
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Several mutations in genes involved in Saccharomyces mating type switching may affect the homothallic behaviour in wine yeasts. In this study the semi-homothallic (Hq) segregation of a flor wine yeast strain was analysed. We aimed to understand the molecular basis of this behaviour in a flor autochthonous strain, verifying the MAT locus status by a PCR-based HO gene disruption and sequencing of the Y region of the HML, HMR and MAT loci, after nested PCR. Presence of ORFs a1 and a2 in the Y region of the HML locus was found. At the ORF a2 at HML locus, a mutation in the stop codon was found, so the a2 ORF contains 33 more bases.
引用
收藏
页码：29 / 36
页数：8
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