Cerebral endothelial nitric oxide synthase expression is reduced after very low flow bypass

被引:5
|
作者
Anttila, Vesa
Christou, Helen
Hagino, Ikuo
Iwata, Yusuke
Mettler, Bret A.
Fernandez-Gonzalez, Angeles
Zurakowski, David
Jonas, Richard A.
机构
[1] Childrens Natl Med Ctr, Dept Cardiovasc Surg, Washington, DC 20010 USA
[2] Univ Hosp Oulu, Dept Surg, Oulu, Finland
[3] Childrens Hosp, Boston, MA 02115 USA
来源
ANNALS OF THORACIC SURGERY | 2006年 / 81卷 / 06期
基金
芬兰科学院;
关键词
D O I
10.1016/j.athoracsur.2006.01.023
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background. In previous studies we have shown that delayed capillary reperfusion after low flow bypass predicts neurologic injury. In this acute study, we hypothesized that low flow reduces endothelial nitric oxide synthase ( eNOS) expression, which may lead to more profound inflammatory response and delayed capillary perfusion. Methods. Twelve piglets (13.2 +/- 0.7 kg) had a cranial window placed over the parietal cerebral cortex for direct examination of the microcirculation using intravital fluorescence microscopy. Animals were cooled to 15 degrees C or 34 degrees C on cardiopulmonary bypass (pH stat, hematocrit 30%, pump flow 100 mL/kg/ minute) followed by 2 hours of low flow (50 mL/kg/minute) or very low flow (10 mL/kg/minute). Rhodamine staining was used to observe adherent and rolling leukocytes in postcapillary venules. The eNOS protein expression was determined by Western immunoblotting. Results. High temperature and low flow rate correlated with significantly reduced eNOS expression (p < 0.01). Univariate comparisons based on Student t tests indicated that eNOS protein levels were lower at 34 C than at 15 degrees C 0.7 +/- 0.6 vs 1.7 +/- 0.5, p < 0.01) and at 10 mL/ kg per minute compared with 50 mL/ kg per minute (0.8 +/- 0.7 vs 1.6 +/- 0.5, p = 0.03). Moreover, two-way analysis of variance revealed that temperature (F = 21.6, p < 0.001) and flow rate (F = 13.8, p = 0.005) were independent multivariate predictors of eNOS expression. During low flow bypass, eNOS was inversely correlated with numbers of adherent (p = 0.002) and rolling (p = 0.006) leukocytes, following an exponential decay curve closely. Conclusions. eNOS expression is reduced after very low flow bypass, particularly at a higher bypass temperature. This is associated with delayed capillary reperfusion. Reduced eNOS is also associated with increased white cell activation which may lead to greater neurologic injury.
引用
收藏
页码:2202 / 2206
页数:5
相关论文
共 50 条
  • [31] Endothelial nitric oxide synthase expression and regulation in mice cerebral arterioles after 4 and 10 weeks of chronic renal failure
    Da Silveira, C.
    Bengrine, A.
    Colangeli, S.
    Poirot, S.
    Massy, Z.
    Chillon, J.
    FUNDAMENTAL & CLINICAL PHARMACOLOGY, 2011, 25 : 49 - 49
  • [32] Nitric Oxide Upregulates Endothelial Nitric Oxide Synthase Expression in Fetal Pulmonary Artery Endothelial Cells • 1783
    Ivan S Yuhanna
    Amy N MacRitchie
    Philip W Shaul
    Pediatric Research, 1998, 43 (Suppl 4) : 304 - 304
  • [33] Transcriptional and posttranscriptional regulation of endothelial nitric oxide synthase expression
    Searles, Charles D.
    AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2006, 291 (05): : C803 - C816
  • [34] Expression of endothelial nitric oxide synthase (eNOS) in the skin microvasculature
    Veves, A
    Quist, WC
    Caballero, EA
    Logerfo, FW
    Horton, ES
    DIABETES, 2000, 49 : A150 - A150
  • [35] Regulation of endothelial nitric oxide synthase expression by Rho GTPase
    Laufs, U
    Liao, JK
    CIRCULATION, 1998, 98 (17) : 313 - 313
  • [36] Effect of nuclear actin on endothelial nitric oxide synthase expression
    Ou, HS
    Shen, YH
    Utama, B
    Wang, J
    Wang, XW
    Coselli, J
    Wang, XL
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2005, 25 (12) : 2509 - 2514
  • [37] Expression of endothelial nitric oxide synthase in hepatocellular carcinomas.
    Lee, KH
    Hon, WM
    Lo, SK
    Khoo, HE
    HEPATOLOGY, 1998, 28 (04) : 344A - 344A
  • [38] Expression of endothelial nitric oxide synthase in ciliated epithelia of rats
    Zhan, XH
    Li, DC
    Johns, RA
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2003, 51 (01) : 81 - 87
  • [39] Expression of endothelial and inducible nitric oxide synthase in human glomerulonephritis
    Furusu, A
    Miyazaki, M
    Abe, K
    Tsukasaki, S
    Shioshita, K
    Sasaki, O
    Miyazaki, K
    Ozono, Y
    Koji, T
    Harada, T
    Sakai, H
    Kohno, S
    KIDNEY INTERNATIONAL, 1998, 53 (06) : 1760 - 1768
  • [40] Effect of nuclear actin on endothelial nitric oxide synthase expression
    Ou, HS
    Shen, YH
    Utama, B
    Wang, J
    Wang, XW
    Coselli, JS
    Wang, XL
    CIRCULATION, 2005, 112 (17) : U223 - U223