Stabilization of d-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles

被引：24
|
作者：
Hsieh, Hao-Chieh [1 ]
Kuan, I-Ching [1 ]
Lee, Shiow-Ling [1 ]
Tien, Gee-Yeng [2 ]
Wang, Yi-Jen [1 ]
Yu, Chi-Yang [1 ]
机构：
[1] Tatung Univ, Dept Bioengn, Taipei 10452, Taiwan
[2] Tatung Univ, Instrument Ctr, Taipei 10452, Taiwan
来源：
BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 04期
关键词：
D-Amino acid oxidase; Immobilization; Magnetic nanoparticle; Rhodosporidium toruloides; Stabilization; HYDROGEN-PEROXIDE; ENZYME;
D O I：
10.1007/s10529-008-9894-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
d-Amino acid oxidase from Rhodosporidium toruloides was immobilized onto glutaraldehyde-activated magnetic nanoparticles. Approximately four enzyme molecules were attached to one magnetic nanoparticle when the weight ratio of the enzyme to the support was 0.12. After immobilization, the T (m) was increased from 45A degrees C of the free form to 55A degrees C. In the presence of 20 mM H2O2, the immobilized form retained 93% of its activity after 5 h while the free form was completely inactivated after 3.5 h.
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页码：557 / 563
页数：7
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