Stabilization of d-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles

被引：24

作者：

Hsieh, Hao-Chieh ^{[1
]}

Kuan, I-Ching ^{[1
]}

Lee, Shiow-Ling ^{[1
]}

Tien, Gee-Yeng ^{[2
]}

Wang, Yi-Jen ^{[1
]}

Yu, Chi-Yang ^{[1
]}

机构：

[1] Tatung Univ, Dept Bioengn, Taipei 10452, Taiwan

[2] Tatung Univ, Instrument Ctr, Taipei 10452, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 04期

关键词：

D-Amino acid oxidase; Immobilization; Magnetic nanoparticle; Rhodosporidium toruloides; Stabilization; HYDROGEN-PEROXIDE; ENZYME;

D O I：

10.1007/s10529-008-9894-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

d-Amino acid oxidase from Rhodosporidium toruloides was immobilized onto glutaraldehyde-activated magnetic nanoparticles. Approximately four enzyme molecules were attached to one magnetic nanoparticle when the weight ratio of the enzyme to the support was 0.12. After immobilization, the T (m) was increased from 45A degrees C of the free form to 55A degrees C. In the presence of 20 mM H2O2, the immobilized form retained 93% of its activity after 5 h while the free form was completely inactivated after 3.5 h.

引用

页码：557 / 563

页数：7

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