Quantitative characterization of light-harvesting efficiency in single molecules and nanoparticles by 2D polarization microscopy: Experimental and theoretical challenges

被引：23

作者：

Camacho, R. ^{[1
]}

Thomsson, D. ^{[1
]}

Yadav, D. ^{[1
]}

Scheblykin, I. G. ^{[1
]}

机构：

[1] Lund Univ, Chem Phys, S-22100 Lund, Sweden

来源：

CHEMICAL PHYSICS | 2012年 / 406卷

基金：

瑞典研究理事会;

关键词：

Light-harvesting; Antenna; Single molecule spectroscopy; Anisotropy; Fluorescence; FRET; Energy funnelling; CONJUGATED POLYMER-MOLECULES; ELECTRONIC-ENERGY TRANSFER; DETECTED CIRCULAR-DICHROISM; FLUORESCENCE MICROSCOPY; HELICENE MOLECULES; LOW-TEMPERATURE; SPECTROSCOPY; COMPLEXES; CHAINS; CHLOROSOMES;

D O I：

10.1016/j.chemphys.2012.03.001

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

General problem of extracting intramolecular energy transfer information from fluorescence and fluorescence excitation polarization experiments at single molecule level is presented. A single funnel approximation is shown to be a very powerful approach to model the polarization data obtained by recently emerged 2-dimensional polarization single molecule imaging technique [O. Mirzov et al., Small 5 (2009) 1877]. Using this approximation a parameter characterising quantitatively light-harvesting efficiency of an individual light-harvesting antenna can be readily obtained. Technical details of 2D polarization imaging and practical methods of avoiding polarization artefact in fluorescence microscopy are discussed. (C) 2012 Elsevier B. V. All rights reserved.

引用

页码：30 / 40

页数：11