Determination of HER2 Amplification Status on Tumour DNA by Digital PCR

被引：28
|
作者：
Garcia-Murillas, Isaac [1 ]
Lambros, Maryou [1 ]
Turner, Nicholas C. [1 ,2 ]
机构：
[1] Inst Canc Res, Breakthrough Breast Canc Res Ctr, London SW3 6JB, England
[2] Royal Marsden Hosp, Breast Unit, London SW3 6JJ, England
来源：
PLOS ONE | 2013年 / 8卷 / 12期
关键词：
POLYMERASE CHAIN-REACTION; BREAST-CANCER; IN-SITU; HYBRIDIZATION; RECEPTOR;
D O I：
10.1371/journal.pone.0083409
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2: EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2: EFTUD2 ratio had high concordance with conventionally defined HER2 status with a sensitivity of 100% (18/18) and a specificity of 98% (57/58). The HER2: EFTUD2 digital PCR assay has potential to accurately assess HER2 amplification status.
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页数：4
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