A filamentous phage of Vibrio parahaemolyticus O3:K6 isolated in Laos

被引：6
|
作者：
Nakasone, N [1 ]
Ikema, M [1 ]
Higa, N [1 ]
Yamashiro, T [1 ]
Iwanaga, M [1 ]
机构：
[1] Univ Ryukyus, Fac Med, Dept Bacteriol, Okinawa 9030215, Japan
来源：
MICROBIOLOGY AND IMMUNOLOGY | 1999年 / 43卷 / 04期
关键词：
filamentous phage; Vibrio parahaemolyticus;
D O I：
10.1111/j.1348-0421.1999.tb02420.x
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
A filamentous phage, 'lvpf5,' of Vibrio parahaemolyticus O3:K6 strain LVP5 was isolated and characterized. The host range was not restricted to serotype O3:K6, but 7 of 99 V. parahaemolyticus strains with a variety of serotypes were susceptible to the phage. The phage was inactivated by heating at 80 C for 10 min and by treating with chloroform. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the phage exhibited a 3.8 kDa protein. The amino-terminal amino acid sequence of the coat protein was determined as AEGGAADPFEAIDLLGVATL. The phage genome consisted of a single-stranded DNA molecule. The activity of the phages was inhibited by anti-Na2 pili antibody.
引用
收藏
页码：385 / 388
页数：4
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