A filamentous phage, 'lvpf5,' of Vibrio parahaemolyticus O3:K6 strain LVP5 was isolated and characterized. The host range was not restricted to serotype O3:K6, but 7 of 99 V. parahaemolyticus strains with a variety of serotypes were susceptible to the phage. The phage was inactivated by heating at 80 C for 10 min and by treating with chloroform. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the phage exhibited a 3.8 kDa protein. The amino-terminal amino acid sequence of the coat protein was determined as AEGGAADPFEAIDLLGVATL. The phage genome consisted of a single-stranded DNA molecule. The activity of the phages was inhibited by anti-Na2 pili antibody.
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Yangzhou Univ, Yangzhou 225009, Peoples R China
Yangzhou Ctr Dis Control & Prevent, Yangzhou 225002, Peoples R ChinaYangzhou Univ, Yangzhou 225009, Peoples R China
Chao, Guoxiang
Wang, Fang
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Yangzhou Univ, Yangzhou 225009, Peoples R ChinaYangzhou Univ, Yangzhou 225009, Peoples R China
Wang, Fang
Zhou, Xiaohui
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Yangzhou Univ, Yangzhou 225009, Peoples R ChinaYangzhou Univ, Yangzhou 225009, Peoples R China
Zhou, Xiaohui
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Jiao, Xinan
Huang, Jinlin
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Yangzhou Univ, Yangzhou 225009, Peoples R ChinaYangzhou Univ, Yangzhou 225009, Peoples R China
Huang, Jinlin
Pan, Zhiming
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Yangzhou Univ, Yangzhou 225009, Peoples R ChinaYangzhou Univ, Yangzhou 225009, Peoples R China
Pan, Zhiming
Zhou, Liping
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Yangzhou Ctr Dis Control & Prevent, Yangzhou 225002, Peoples R ChinaYangzhou Univ, Yangzhou 225009, Peoples R China
Zhou, Liping
Qian, Xiaoqin
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Yangzhou Ctr Dis Control & Prevent, Yangzhou 225002, Peoples R ChinaYangzhou Univ, Yangzhou 225009, Peoples R China