Next-Generation Sequencing-Based Genome-Wide Mutation Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300 Strain

被引:12
|
作者
Lee, Chang-Soo [1 ]
Nam, Jae-Young [1 ]
Son, Eun-Suk [1 ]
Kwon, O-chul [1 ]
Han, Woorijarang [1 ]
Cho, Jae-Yong [2 ]
Park, Young-Jin [1 ]
机构
[1] Konkuk Univ, Dept Appl Biochem, Chungju 380701, South Korea
[2] Sangji Univ, Div Anim Sci & Biotechnol, Wonju 220702, South Korea
关键词
mutation; SNP; InDel; genome; Corynebacterium glutamicum; MUTANT;
D O I
10.1007/s12275-012-2109-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In order to identify single nucleotide polymorphism and insertion/deletion mutations, we performed whole-genome re-sequencing of the enhanced L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain. In total, 142 single nucleotide polymorphisms and 477 insertion/deletion mutations were identified in the ATCC 21300 strain when compared to 3,434 predicted genes of the wild-type C. glutamicum ATCC 13032 strain. Among them, 110 transitions and 29 transversions of single nucleotide polymorphisms were found from genes of the ATCC 21300 strain. In addition, 11 genes, involved in the L-lysine biosynthetic pathway and central carbohydrate metabolism, contained mutations including single nucleotide polymorphisms and insertions/deletions. Interestingly, RT-PCR analysis of these 11 genes indicated that they were normally expressed in the ATCC 21300 strain. This information of genome-wide gene-associated variations will be useful for genome breeding of C. glutamicum in order to develop an industrial amino acid-producing strain with minimal mutation.
引用
收藏
页码:860 / 863
页数:4
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