NS1 antigen: A new beam of light in the early diagnosis of dengue infection

被引：9
|
作者：
Suleman, Muhammad [1 ,2 ]
Faryal, Rani [1 ]
Alam, Muhammad Masroor [2 ]
Sharif, Salmaan [2 ]
Shaukat, Shahzad [2 ]
Aamir, Uzma Bashir [2 ]
Khurshid, Adnan [2 ]
Angez, Mehar [2 ]
Umair, Massab [2 ]
Sufian, Mian Muhammad [2 ]
Arshad, Yasir [2 ]
Mujtaba, Ghulam [2 ]
Zaidi, Syed Sohail Zahoor [2 ]
机构：
[1] Quaid I Azam Univ, Dept Microbiol, Islamabad, Pakistan
[2] Natl Inst Hlth, Dept Virol, Pk Rd, Islamabad 45500, Pakistan
来源：
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2016年 / 9卷 / 12期
关键词：
Dengue; Pakistan; Epidemiology; ELISA; NS1; Antigen; PCR;
D O I：
10.1016/j.apjtm.2016.10.007
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Objective: To evaluate NS1 antigen detection ELISA for the early laboratory diagnosis of dengue virus infection. Methods: The present study was conducted to evaluate the overall positivity of NS1 antigen detection ELISA and its comparison with viral RNA detection via real time PCR and IgM antibodies detection by ELISA. Results: A total of 1 270 serum samples were tested 86% (1 097/1 270) were detected positive by one or more than one diagnostic test. Out of 1 270, 64% (807/1 270) were positive by NS1 ELISA and 52% (662/1 270), 51% (646/1 270) were positive by real-time RT-PCR and IgM ELISA respectively. Conclusions: NS1 antigen detection ELISA is highly suitable diagnostic tools and it also has great value for use in outbreak and epidemic situation.
引用
收藏
页码：1187 / 1189
页数：3
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