Real-Time Analysis and Visualization for Single-Molecule Based Super-Resolution Microscopy

被引：100
|
作者：
Kechkar, Adel [1 ,2 ]
Nair, Deepak [1 ,2 ]
Heilemann, Mike [3 ]
Choquet, Daniel [1 ,2 ]
Sibarita, Jean-Baptiste [1 ,2 ]
机构：
[1] Univ Bordeaux, Interdisciplinary Inst Neurosci, Bordeaux, France
[2] CNRS, UMR 5297, Bordeaux, France
[3] Goethe Univ Frankfurt, Inst Phys & Theoret Chem, Frankfurt, Germany
来源：
PLOS ONE | 2013年 / 8卷 / 04期
关键词：
PHOTOACTIVATION LOCALIZATION MICROSCOPY; OPTICAL RECONSTRUCTION MICROSCOPY; ACCURATE; SOFTWARE; TRACKING;
D O I：
10.1371/journal.pone.0062918
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy. We demonstrate a functional method for real-time reconstruction with automatic feedback control, without compromising the localization accuracy. Compatible with high frame rates of EM-CCD cameras, it relies on a wavelet segmentation algorithm, together with a mix of CPU/GPU implementation. A combination with Gaussian fitting allows direct access to 3D localization. Automatic feedback control ensures optimal molecule density throughout the acquisition process. With this method, we significantly improve the efficiency and feasibility of localization-based super-resolution microscopy.
引用
收藏
页数：10
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