In vitro effect of recombinant human granulocyte colony-stimulating factor on canine neutrophil apoptosis

被引:4
|
作者
Oguma, K
Sano, J
Kano, R
Watari, T
Moritomo, T
Hasegawa, A
机构
[1] Nihon Univ, Sch Vet Med, Dept Pathobiol, Fujisawa, Kanagawa 2528510, Japan
[2] Nihon Univ, Sch Vet Med, Comprehens Vet Clin Studies, Fujisawa, Kanagawa 2528510, Japan
[3] Nihon Univ, Sch Vet Med, Dept Fish Pathol, Fujisawa, Kanagawa 2528510, Japan
关键词
dog; neutrophil; apoptosis; G-CSF;
D O I
10.1016/j.vetimm.2005.06.003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Apoptosis is essential in eliminating neutrophils (polymorphonuclear leukocytes: PMNs) in animals. The suppression of PMN apoptosis is believed to be beneficial in eradicating pathogens and is implicated in the pathogenesis of human inflammatory diseases. In the present study, canine PMNs were stimulated with recombinant human granulocyte colonystimulating factor (rhG-CSF) to investigate the in vitro effect on the apoptosis of canine PMNs. Apoptotic cell rates were assessed by flow cytometry in relation to the ability of PMNs to produce reactive oxygen species (ROS). Canine PMN apoptosis was markedly suppressed by rhG-CSF treatment, in association with the retention of the PMN ability to produce ROS. The addition of cycloheximide abolished this suppression by rhG-CSF. Moreover, canine PMNs, which were stimulated by rhG-CSF, expressed high levels of anti-apoptotic mcl-1 gene mRNA, as quantified by real-time polymerase chain reaction method. The results suggest that PMNs, stimulated by G-CSF, could work effectively over a longer period to eliminate pathogens, and that the prolongation of the PMN life-span might occasionally aggravate tissue injuries in dogs. In addition, the suppression of PMN apoptosis seems to be mediated by the induction of anti-apoptotic mcl-1 gene expression. (c) 2005 Published by Elsevier B.V.
引用
收藏
页码:307 / 314
页数:8
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