Combined optical coherence and two-photon excitation microscopy

被引:0
|
作者
Beaurepaire, E [1 ]
Moreaux, L [1 ]
Mertz, J [1 ]
机构
[1] Ecole Super Phys & Chim Ind Ville Paris, Lab Neurophysiol & Nouvelles Microscopies, INSERM EPI 00 02, F-75005 Paris, France
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中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We demonstrate simultaneous imaging by optical coherence microscopy (OCM) and two-photon-excited fluorescence (TPEF) microscopy. Independent contrast mechanisms derived from fluorescence and backscattered light provide complementary information on biological tissues. The coupling of OCM and TPEF microscopy is natural in that the two imaging modes may be run off the same laser source. The confocal arrangement for OCM provides a sub-micrometer resolution compatible with TPEF microscopy.
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页码:43 / 48
页数:6
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