Effect of Bt Broccoli or Plants Treated With Insecticides on Ovipositional Preference and Larval Survival of Plutella xylostella (Lepidoptera: Plutellidae)

The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a major pest of cruciferous crops throughout the world, has demonstrated an ability to develop resistance to many different classes of insecticides, including proteins from Bacillus thuringiensis that are expressed in plants (Bt plants). The ovipositional preferences and larval survival of strains (resistant strain, RR; heterozygous strain, RS; susceptible strain, SS) of P. xylostella to Cry1Ac-expressing broccoli or broccoli plants treated with lambda-cyhalothrin or spinosad were studied under greenhouse condition. Numbers of eggs per plant did not differ between Bt broccoli and non-Bt broccoli for Bt-RR, Bt-RS, and Bt-SS adults. Ovipositing adults (spinosad-RR, spinosad-RS, and spinosad-SS) also could not discriminate between spinosad-treated and untreated plants, and oviposition did not increase over the 13 d after spinosad treatment. For broccoli treated with lambda-cyhalothrin at the diagnostic dose of 20 ppm, all three insect strains (lc-RR, lc-RS, and lc-SS) had constant oviposition over time based on linear regressions. At the field dose of 80 ppm, the lc-RR strain had constant oviposition over time. The lc-SS susceptible strain had increasing oviposition over time, but the oviposition pattern on the nonsprayed broccoli also increased over time. Susceptible females layed fewer eggs on plants sprayed with lambda-cyhalothrin than on unsprayed plants. A residue-persistence test showed that spinosad and lambda-cyhalothrin could effectively control SS P. xylostella larvae for 7-9 d after application. These results are discussed in relation to their potential impact on insecticide resistance management strategies.