SIMULTANEOUS IMAGING OF A lacZ-MARKED TUMOR AND MICROVASCULATURE MORPHOLOGY IN VIVO BY DUAL-WAVELENGTH PHOTOACOUSTIC MICROSCOPY

被引:38
|
作者
Li, Li [1 ]
Zhang, Hao F. [1 ,2 ]
Zemp, Roger J. [1 ,3 ]
Maslov, Konstantin [1 ]
Wang, Lihong V. [1 ]
机构
[1] Washington Univ, Opt Imaging Lab, Dept Biomed Engn, St Louis, MO 63130 USA
[2] Univ Wisconsin, Dept Elect Engn, Milwaukee, WI 53201 USA
[3] Univ Alberta, Dept Elect & Comp Engn, Edmonton, AB T6G2V4, Canada
关键词
Photoacoustic; molecular imaging; gene expression; reporter gene;
D O I
10.1142/S1793545808000212
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme beta-galactosidase. Dual-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo, with a superior resolution on the order of 10 mu m. Tumor-feeding vessels were found, and the expression level of lacZ in tumor was estimated. With future development of new absorption-enhancing reporter-gene systems, we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation, progression, and metastasis, and in its response to therapy.
引用
收藏
页码:207 / 215
页数:9
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