Disodium Salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) Phenyl Phosphate as a Fluorescent Probe for Determination of ALP

被引：2
|
作者：
Lei Yingjie [1 ]
Ouyang Jie [1 ]
Ding Mei [1 ]
机构：
[1] Tianjin Univ Technol, Sch Chem & Chem Engn, Tianjin, Peoples R China
来源：
APPLIED MECHANICS AND MECHANICAL ENGINEERING II, PTS 1 AND 2 | 2012年 / 138-139卷
关键词：
ALP; fluorescent probe; 2-(5-phenyl-1,3,4-oxadiazol-2-yl)phenol; synthesis; FLUOROMETRIC-DETERMINATION; HUMAN SERUM; ALKALINE; SUBSTRATE;
D O I：
10.4028/www.scientific.net/AMM.138-139.1109
中图分类号：
TH [机械、仪表工业];
学科分类号：
0802 ;
摘要：
A convenient method to synthesize disodium salt of 2-(5-phenyl-1, 3, 4-oxadiazol-2-yl) phenyl phosphate (OXDP), a substrate for the fluorescent determination of alkaline phosphatase (ALP) activity, via an one-pot procedure of condensation between the benzoic acid and 2-hydroxybenzhydrazide at triethylamine alkaline solution in the presence of propylphosphonic anhydride, was realized. Preliminary tests reveal that the lessened fluorescence intensity resulting from enzyme reaction is proportional to ALP activity in the range of 1.0-25.0 U/L with the detection limit of 0.25U/L, which can be successfully applied to assess ALP in human serum with good precision.
引用
收藏
页码：1109 / 1114
页数：6
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