A unique caleosin in oil bodies of lily pollen

被引：21
|
作者：
Jiang, Pei-Luen [1 ]
Jauh, Guang-Yuh [2 ]
Wang, Co-Shing [1 ]
Tzen, Jason T. C. [1 ,3 ]
机构：
[1] Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 402, Taiwan
[2] Acad Sinica, Inst Plant & Microbial Biol, Taipei 115, Taiwan
[3] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan
来源：
PLANT AND CELL PHYSIOLOGY | 2008年 / 49卷 / 09期
关键词：
caleosin; lily (Lilium longiflorum thunb.); oil bodies; oleosin; pollen;
D O I：
10.1093/pcp/pcn103
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.
引用
收藏
页码：1390 / 1395
页数：6
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