Isolation and characterization of first microsatellite markers for the noble crayfish, Astacus astacus

被引：15

作者：

Koiv, Kuldar ^{[1
]}

Gross, Riho ^{[1
]}

Paaver, Tiit ^{[1
]}

Kuehn, Ralph ^{[2
]}

机构：

[1] Estonian Univ Life Sci, Inst Vet Med & Anim Sci, Dept Aquaculture, EE-51014 Tartu, Estonia

[2] Tech Univ Munich, Dept Anim Sci, D-85354 Freising Weihenstephan, Germany

来源：

CONSERVATION GENETICS | 2008年 / 9卷 / 06期

关键词：

Microsatellite loci; SSR; Population structure; Genetic diversity; Astacus astacus;

D O I：

10.1007/s10592-008-9539-8

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Noble crayfish (Astacus astacus) is listed as vulnerable by the IUCN Red List of Threatened Species in Europe. However, very little is known about genetic diversity and structuring of noble crayfish populations, mainly because of the lack of informative genetic markers. We describe the isolation and characterization of the first microsatellite markers for this species, which were obtained by screening 4,000 recombinant clones. Eight loci revealed polymorphisms in a panel of 172 individuals from seven populations in Northern Europe. Number of alleles per locus ranged from two to 10 (average 4.4) and heterozygosity levels among populations varied from 0 to 0.80 for H (o) and from 0 to 0.72 for H-e.

引用

页码：1703 / 1706

页数：4

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