Study of the reaction between nucleic acids and Tb-BPMPHD-CTMAB complex and its analytical application

被引：12
|
作者：
Wu, X [1 ]
Yang, JH [1 ]
Huang, F [1 ]
Wang, M [1 ]
Sun, LM [1 ]
Xu, GY [1 ]
机构：
[1] Shandong Univ, Coll Chem, Lab Colloid & Interface Chem Educ Minist, Jinan 250100, Peoples R China
来源：
ANALYTICAL LETTERS | 1999年 / 32卷 / 12期
关键词：
nucleic acids; fluorescence quenching; Tb-BPMPHD-CTMAB complex;
D O I：
10.1080/00032719908542978
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The fluorescence quenching of the Tb-BPMPHD-CTMAB by nucleic acids is reported. The maximum fluorescence quenching was obtained at pH5.5, with maximum excitation and emission wavelengths at 300 and 543nm, respectively. Under optimal conditions, the differential value of fluorescence intensity between the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range of 4.0x10(-8) ~ 1.0x10(-5) g/ml for fsDNA and 2.5x10(-8) ~ 6.15x10(-6) g/ml for yRNA, respectively. The corresponding detection limits are 9.0ng/ml for fsDNA and 5.0ng/ml for yRNA. The method was applied to the determination of DNA (or RNA) in synthetic samples. The results obtained were satisfactory.
引用
收藏
页码：2417 / 2425
页数：9
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