Separation and identification of corticosterone metabolites by liquid chromatography electrospray ionization mass spectrometry

被引：24
|
作者：
Miksík, I
Vylitová, M
Pácha, J
Deyl, Z
机构：
[1] Acad Sci Czech Republ, Inst Physiol, CZ-14220 Prague 4, Czech Republic
[2] Charles Univ, Sch Med 2, Dept Physiol, CZ-15000 Prague, Czech Republic
来源：
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 726卷 / 1-2期
关键词：
corticosterone;
D O I：
10.1016/S0378-4347(99)00028-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-performance liquid chromatography coupled to atmospheric pressure ionization-electrospray ionization mass spectrometry (API-ESI-MS) was investigated for the analysis of corticosterone metabolites; their characterization was obtained by combining the separation on Zorbax Eclipse XDB C-18 column (eluted with a methanol-water-acetic acid gradient) with identification using positive ion mode API-ESI-MS and selected ion analysis. The applicability of this method was verified by monitoring the activity of steroid converting enzymes (20 beta-hydroxysteroid dehydrogenase and 11 beta-hydroxysteroid dehydrogenase) in avian intestines. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：59 / 69
页数：11
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