Immobilization of urease on vermiculite

被引：20
|
作者：
Anita, A [1 ]
Sastry, CA [1 ]
Hashim, MA [1 ]
机构：
[1] UNIV MALAYA,INST ADV STUDIES,KUALA LUMPUR 50603,MALAYSIA
来源：
BIOPROCESS ENGINEERING | 1997年 / 16卷 / 06期
关键词：
D O I：
10.1007/s004490050338
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Urease (EC 3.5.1.5) of high activity was obtained 2.2 when the enzyme was immobilized on vermiculite crosslinked with 2.5% glutaraldehyde in chilled EDTA-phosphate buffer (pH 5.5). The highest activity of the immobilized enzyme was at 65 degrees C and PH 6.5 while the optimum temperature for free urease was found to be 25 degrees C. The thermal stability of immobilized urease was observed to be much better than that of the free urease. When stored at 4 degrees C, urease immobilized on vermiculite retained 69 to 81% of its activity after 60 days and 61 to 75% of its original activity was retained after 4 repeated uses.
引用
收藏
页码：375 / 380
页数：6
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