Production of Polyhydroxybutyrate by Bacillus axaraqunsis BIPC01 using Petrochemical Wastewater as Carbon Source

被引：19

作者：

Mayeli, Nasim ^{[1
]}

Motamedi, Hossein ^{[1
]}

Heidarizadeh, Fariba ^{[2
]}

机构：

[1] Shahid Chamran Univ Ahvaz, Dept Biol, Fac Sci, Ahvaz, Iran

[2] Shahid Chamran Univ Ahvaz, Dept Chem, Fac Sci, Ahvaz, Iran

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2015年 / 58卷 / 04期

关键词：

polyhydroxyalkanoate; petrochemical wastewater; Bacillus axaraqunsis; biodegradable polymer; bioplastic; PHB; POLYHYDROXYALKANOATES;

D O I：

10.1590/S1516-8913201500048

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The aim of this study was to use petrochemical wastewater as the source of carbon for the production of polyhydroxyalkanoates (PHA) in an effort to decrease its cost of production. For this purpose, PHA producing bacteria were isolated from the petrochemical wastewater of Bandar Imam, Iran. The purified colonies were screened for PHA by Sudan Black B and Nile Blue A staining. Among positively stained bacteria, the best PHA producer was selected on the basis of cell growth, PHA content and the monomer composition of PHA. The phenotypic and genotypic identification this isolate showed it to be Bacillus axaraqunsis. The PHA was produced at a cell density of about 9.46 g/l of maximum concentration of 6.33g/l (l), corresponding to 66% of cell dry weight. These results showed that B. axaraqunsis BIPC01 could be a potent PHA producer using wastewater for industrial purpose and simultaneously reducing the environmental pollution.

引用

页码：643 / 650

页数：8

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