Isobavachalcone Induces Multiple Cell Death in Human Triple-Negative Breast Cancer MDA-MB-231 Cells

被引:12
|
作者
Wu, Cheng-Zhu [1 ,2 ]
Gao, Mei-Jia [1 ]
Chen, Jie [1 ]
Sun, Xiao-Long [1 ]
Zhang, Ke-Yi [1 ]
Dai, Yi-Qun [1 ]
Ma, Tao [1 ]
Li, Hong-Mei [1 ,2 ]
Zhang, Yu-Xin [2 ,3 ]
机构
[1] Bengbu Med Coll, Sch Pharm, 2600 Donghai Rd, Bengbu 233030, Peoples R China
[2] Anhui Prov Biochem Pharmaceut Engn Technol Res Ct, Bengbu 233030, Peoples R China
[3] Bengbu Med Coll, Sch Lab Med, 2600 Donghai Rd, Bengbu 233030, Peoples R China
来源
MOLECULES | 2022年 / 27卷 / 20期
关键词
TNBC; natural product; isobavachalcone; cell death; necroptosis; RIP3; DRUG-RESISTANCE; APOPTOSIS; NECROPTOSIS; FLAVONOIDS; INDUCTION; RIP3;
D O I
10.3390/molecules27206787
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Standardized treatment guidelines and effective drugs are not available for human triple-negative breast cancer (TNBC). Many efforts have recently been exerted to investigate the efficacy of natural compounds as anticancer agents owing to their low toxicity. However, no study has examined the effects of isobavachalcone (IBC) on the programmed cell death (PCD) of human triple-negative breast MDA-MB-231 cancer cells. In this study, IBC substantially inhibited the proliferation of MDA-MB-231 cells in concentration- and time-dependent manners. In addition, we found that IBC induced multiple cell death processes, such as apoptosis, necroptosis, and autophagy in MDA-MB-231 cells. The initial mechanism of IBC-mediated cell death in MDA-MB-231 cells involves the downregulation of Akt and p-Akt-473, an increase in the Bax/Bcl-2 ratio, and cleaved caspases-3 induced apoptosis; the upregulation of RIP3, p-RIP3 and MLKL induced necroptosis; as well as a simultaneous increase in LC3-II/I ratio induced autophagy. In addition, we observed that IBC induced mitochondrial dysfunction, thereby decreasing cellular ATP levels and increasing reactive oxygen species accumulation to induce PCD. These results suggest that IBC is a promising lead compound with anti-TNBC activity.
引用
收藏
页数:16
相关论文
共 50 条
  • [41] Miconazole triggers various forms of cell death in human breast cancer MDA-MB-231 cells
    Wu, Chengzhu
    Gao, Meijia
    Shen, Lin
    Li, Bohan
    Bai, Xiangjian
    Gui, Jiahui
    Li, Hongmei
    Huo, Qiang
    Ma, Tao
    PHARMAZIE, 2019, 74 (05): : 290 - 294
  • [42] Moderate intermittent negative pressure increases invasiveness of MDA-MB-231 triple negative breast cancer cells
    Liu, Wenyue
    Fu, Xin
    Yang, Zhigang
    Li, Shangshan
    Cao, Yan
    Li, Qiuchen
    Luan, Jie
    BREAST, 2018, 38 : 14 - 21
  • [43] The anti-migration and anti-invasion effects of Bruceine D in human triple-negative breast cancer MDA-MB-231 cells
    Luo, Can
    Wang, Yu
    Wei, Cheng
    Chen, Yuxin
    Ji, Zhaoning
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2020, 19 (01) : 273 - 279
  • [44] Atorvastatin induces downregulation of matrix metalloproteinase-2/9 in MDA-MB-231 triple negative breast cancer cells
    Bakar-Ates, Filiz
    Ozkan, Erva
    MEDICAL ONCOLOGY, 2022, 40 (01)
  • [45] Thioglucose-bound gold nanoparticles increase the radiosensitivity of a triple-negative breast cancer cell line (MDA-MB-231)
    Wang, Cuihong
    Jiang, Yuhua
    Li, Xiaohong
    Hu, Likuan
    BREAST CANCER, 2015, 22 (04) : 413 - 420
  • [46] Lapatinib as a Dual Tyrosine Kinase Inhibitor Unexpectedly Activates Akt in MDA-MB-231 Triple-Negative Breast Cancer Cells
    Kaboli, Parham Jabbarzadeh
    Ling, King-Hwa
    LETTERS IN DRUG DESIGN & DISCOVERY, 2020, 17 (08) : 1060 - 1063
  • [47] Thioglucose-bound gold nanoparticles increase the radiosensitivity of a triple-negative breast cancer cell line (MDA-MB-231)
    Cuihong Wang
    Yuhua Jiang
    Xiaohong Li
    Likuan Hu
    Breast Cancer, 2015, 22 : 413 - 420
  • [48] Extracts and components of Ficus carica leaves suppress survival, cell cycle, and migration of triple-negative breast cancer MDA-MB-231 cells
    Zhang, Yu
    Wan, Youzhong
    Hu, Bo
    Li, Boyuan
    Jin, Yue
    Hu, Xin
    ONCOTARGETS AND THERAPY, 2018, 11 : 4377 - 4386
  • [49] Dillenia suffruticosa dichloromethane root extract induced apoptosis towards MDA-MB-231 triple-negative breast cancer cells
    Foo, Jhi Biau
    Yazan, Latifah Saiful
    Tor, Yin Sim
    Wibowo, Agustono
    Ismail, Norsharina
    Armania, Nurdin
    Cheah, Yoke Kqueen
    Abdullah, Rasedee
    JOURNAL OF ETHNOPHARMACOLOGY, 2016, 187 : 195 - 204
  • [50] Resveratrol is an inhibitor of sodium-dependent inorganic phosphate transport in triple-negative MDA-MB-231 breast cancer cells
    Lacerda-Abreu, Marco Antonio
    Russo-Abrahao, Thais
    Meyer-Fernandes, Jose Roberto
    CELL BIOLOGY INTERNATIONAL, 2021, 45 (08) : 1768 - 1775