Development of colloidal gold-based immunochromatographic assay for rapid detection of Alicyclobacillus acidoterrestris in apple juice

The colloidal gold quick test card which we developed was used for rapid detection of Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice concentrate. Two antibodies against A. acidoterrestris, obtained from Sprague Dawley (SD) rats and Japan White Rabbits, were adopted to construct this quick test card. The results showed that the colloidal gold quick test card had better specificity, and was more rapid and convenient which deserved notice especially (the test could be completed within 5-10 min) and concorded with ELISA method (we previously report) and K medium methods (traditional classical method). This new developed colloidal gold quick test card (we named it TAB quick test card) could be more convenient for apple juice safety testing. RESUMENSe utilizo la tarjeta de test rapido de oro coloidal que desarrollamos para la deteccion rapida de Alicyclobacillus acidoterrestris (A. acidoterrestris) en concentrado de zumo de manzana (AJC), dos anticuerpos contra A. acidoterrestris que se obtuvieron de ratas Sprague Dawley (SD) y conejos blancos de Japon se utilizaron para realizar esta tarjeta de test rapido. Los resultados mostraron que tenian una mejor especificidad, eran mas rapidas y practicas, ademas de merecer una especial atencion (el test podia ser completado en 5-10 min) y una buena concordancia con el metodo ELISA (estudiado anteriormente) y los metodos K-medias (metodo clasico tradicional). Esta nueva tarjeta de test rapido de oro coloidal desarrollada (nombrada tarjeta del test rapido TAB) podria ser mas practica para las pruebas de seguridad del zumo de manzana.