Electrofusion of giant unilamellar vesicles to cells

被引：6
|
作者：
Aroush, Dikla Raz-Ben [1 ]
Yehudai-Resheff, Shlomit [1 ,4 ]
Keren, Kinneret [1 ,2 ,3 ]
机构：
[1] Technion Israel Inst Technol, Dept Phys, IL-32000 Haifa, Israel
[2] Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, Haifa, Israel
[3] Technion Israel Inst Technol, Network Biol Res Labs, Haifa, Israel
[4] Rambam Healthcare Campus, Dept Hematol, Haifa, Israel
来源：
BIOPHYSICAL METHODS IN CELL BIOLOGY | 2015年 / 125卷
关键词：
MEMBRANE-FUSION; MECHANISMS; TENSION; ELECTROPORATION; EXOCYTOSIS; SHAPE;
D O I：
10.1016/bs.mcb.2014.11.005
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We describe a method for electroporation-induced fusion of giant unilamellar vesicles (GUVs) with the plasma membrane of adherent cells. Using this method, the area of the cell membrane can be abruptly increased and various lipids can be introduced into the membrane. The process involves two steps: (1) the formation of GUVs with controlled membrane composition and (2) electrofusion of the GUVs to living cells using an electroporator for adherent cells. We demonstrate the technique on fish epithelial keratocytes and human foreskin fibroblasts, and discuss the influence of the composition of the GUVs on the fusion process.
引用
收藏
页码：409 / 422
页数：14
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