Isolation of chlorophylls a and b from spinach by counter-current chromatography

被引：36
|
作者：
Jubert, Carole
Bailey, George
机构：
[1] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA
[2] Oregon State Univ, Environm Hlth Sci Ctr, Corvallis, OR 97331 USA
[3] Oregon State Univ, Marine Freshwater Biomed Sci Ctr, Corvallis, OR 97331 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1140卷 / 1-2期
关键词：
counter-current chromatography; chlorophylls a and b;
D O I：
10.1016/j.chroma.2006.11.063
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for the isolation of chlorophylls from spinach by counter-current chromatography was developed. An initial extraction protocol was devised to avoid the notorious sensitivity of chlorophylls to degradation by light, heat, oxygen, acids and bases. Further purification and separation of chlorophylls a and b were achieved using counter-current chromatography. Chlorophyll structures and purities were established by HPLC, fast atom bombardment mass spectrometry and nuclear magnetic resonance. Purity was estimated to be > 95% (100% by HPLC). Typical yields from 30 g of freeze-dried spinach were 300 mg of chlorophyll a and 100 mg of chlorophyll b. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：95 / 100
页数：6
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