Detection of shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in eastern Slovakia

被引：6
|
作者：
Liptáková, A
Siegfried, L
Podracká, L
Sabol, M
Sehnálková, H
Bogyiová, E
Rosocha, J
Kmetová, M
Kerestesová, H
Kotulova, D
机构：
[1] Safarik Univ, Fac Med, Inst Med Microbiol, Kosice, Slovakia
[2] Fac Hosp, Pediat Clin, Kosice, Slovakia
[3] Fac Hosp, Dept Microbiol, Kosice, Slovakia
[4] Safarik Univ, Fac Med, Associated Tissue Bank, Kosice, Slovakia
[5] Comenius Univ, Fac Med, Inst Microbiol, Bratislava, Slovakia
来源：
FOLIA MICROBIOLOGICA | 2002年 / 47卷 / 02期
关键词：
D O I：
10.1007/BF02817680
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin I and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin I and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.
引用
收藏
页码：185 / 188
页数：4
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