Detection of shiga toxins, intimin and enterohemolysin in Escherichia coli strains isolated from children in eastern Slovakia

被引：6
|
作者：
Liptáková, A
Siegfried, L
Podracká, L
Sabol, M
Sehnálková, H
Bogyiová, E
Rosocha, J
Kmetová, M
Kerestesová, H
Kotulova, D
机构：
[1] Safarik Univ, Fac Med, Inst Med Microbiol, Kosice, Slovakia
[2] Fac Hosp, Pediat Clin, Kosice, Slovakia
[3] Fac Hosp, Dept Microbiol, Kosice, Slovakia
[4] Safarik Univ, Fac Med, Associated Tissue Bank, Kosice, Slovakia
[5] Comenius Univ, Fac Med, Inst Microbiol, Bratislava, Slovakia
来源：
FOLIA MICROBIOLOGICA | 2002年 / 47卷 / 02期
关键词：
D O I：
10.1007/BF02817680
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin I and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin I and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.
引用
收藏
页码：185 / 188
页数：4
相关论文
共 50 条
  • [1] Detection of Shiga toxins, intimin and enterohemolysin inEscherichia coli strains isolated from children in Eastern Slovakia
    A. Liptákova
    L. Siegfried
    L'. Podracká
    M. Sabol
    H. Sehnálková
    E. Bogyiová
    J. Rosocha
    M. Kmeťová
    H. Kerestešová
    D. Kotulová
    Folia Microbiologica, 2002, 47 : 185 - 188
  • [2] Detection of Enterohemolysin and Intimin Genes in Escherichia coli O157:H7 Strains Isolated from Calves and Cattle in Afyonkarahisar - Turkey
    Kuyucuoglu, Yahya
    Seker, Esra
    Sareyyupoglu, Baris
    Gurler, Zeki
    KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, 2011, 17 (04) : 663 - 666
  • [3] Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli
    Reischl, U
    Youssef, MT
    Kilwinski, J
    Lehn, N
    Zhang, WL
    Karch, H
    Strockbine, NA
    JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (07) : 2555 - 2565
  • [4] PCR Detection of Shiga Toxins, Enterohaemolysin and Intimin Virulence Genes of Escherichia coli O157:H7 Strains Isolated from Faeces of Anatolian Water Buffaloes in Turkey
    Seker, E.
    Kuyucuoglu, Y.
    Sareyyupoglu, B.
    Yardimci, H.
    ZOONOSES AND PUBLIC HEALTH, 2010, 57 (7-8) : E33 - E37
  • [5] Detection and source tracking of Escherichia coli, harboring intimin and Shiga toxin genes, isolated from the Little Bighorn River, Montana
    Hamner, Steve
    Broadaway, Susan C.
    Berg, Ethan
    Stettner, Sean
    Pyle, Barry H.
    Man, Nita Big
    Elk, Joseph Old
    Eggers, Margaret J.
    Doyle, John
    Kindness, Larry
    Luck, Brandon Good
    Ford, Timothy E.
    Camper, Anne C.
    INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH, 2014, 24 (04) : 341 - 362
  • [6] Identification of enteropathogenic and enterohaemorrhagic Escherichia coli strains by immunoserological detection of intimin
    Menezes, M. A.
    Rocha, L. B.
    Koga, P. C. M.
    Fernandes, I.
    Nara, J. M.
    Magalhaes, C. A.
    Abe, C. M.
    Ayala, C. O.
    Burgos, Y. K.
    Elias, W. P.
    Castro, A. F. P.
    Piazza, R. M. F.
    JOURNAL OF APPLIED MICROBIOLOGY, 2010, 108 (03) : 878 - 887
  • [7] Shiga toxin Genes from Escherichia coli strains isolated from mastitic milk
    Cursons, RT
    Williamson, J
    Bean, A
    MASTITIS IN DAIRY PRODUCTION: CURRENT KNOWLEDGE AND FUTURE SOLUTIONS, 2005, : 671 - 676
  • [8] CLOSE ASSOCIATION OF VEROTOXIN (SHIGA-LIKE TOXIN) PRODUCTION WITH ENTEROHEMOLYSIN PRODUCTION IN STRAINS OF ESCHERICHIA-COLI
    BEUTIN, L
    MONTENEGRO, MA
    ORSKOV, I
    ORSKOV, F
    PRADA, J
    ZIMMERMANN, S
    STEPHAN, R
    JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (11) : 2559 - 2564
  • [9] Serotypes, virulence genes, intimin types and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia
    Vu-Khac, H.
    Holoda, E.
    Pilipcinec, E.
    Blanco, M.
    Blanco, J. E.
    Dahbi, G.
    Mora, A.
    Lopez, C.
    Gonzalez, E. A.
    Blanco, J.
    VETERINARY JOURNAL, 2007, 174 (01): : 176 - 187
  • [10] Prevalence of shiga toxin genes among Escherichia coli strains isolated from pigs
    Osek, J
    VETERINARY RECORD, 1999, 145 (19) : 557 - 558
12345