Differential expression of members of the IGF system in OPU-derived oocytes from Nelore (Bos indicus) and Holstein (Bos taurus) cows

被引：24
|
作者：
Satrapa, Rafael A. [1 ]
Castilho, Anthony S. [1 ]
Razza, Eduardo M. [1 ]
Pegorer, Marcelo F. [1 ]
Puelker, Raquel [1 ]
Barros, Ciro M. [1 ]
机构：
[1] Univ Sao Paulo State, Dept Pharmacol, Botucatu, SP, Brazil
来源：
ANIMAL REPRODUCTION SCIENCE | 2013年 / 138卷 / 3-4期
基金：
巴西圣保罗研究基金会;
关键词：
Bovine; OPU; Oocyte; IGF; IGFR; PAPP-A; GROWTH-FACTOR-I; MESSENGER-RNA TRANSCRIPTS; FACTOR BINDING PROTEIN-2; BOVINE EMBRYOS; PROTEOLYTIC DEGRADATION; BLASTOCYST DEVELOPMENT; NUCLEAR MATURATION; FOLLICLE SELECTION; OVARIAN-FOLLICLES; FACTOR RECEPTORS;
D O I：
10.1016/j.anireprosci.2013.02.023
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The insulin-like growth factor (IGF) system is related to quality of oocytes and embryos. The aim of this study was to investigate the mRNA levels of IGF1 and IGF2 and their receptors, IGFR1 and IGFR2, as well as IGFBP2, IGFBP4, and PAPP-A in oocytes from Nelore compared to Holstein cows. Pools of oocytes (20 oocytes/pool) from Nelore (n = 8 pools) and Holstein (n = 4 pools) were obtained via ovum pick-up (OPU, 10 sessions) and cumulus cells and zona pellucida were removed. The pools were submitted to total RNA extraction. Expression of members of the IGF system was assessed by real time RT-PCR. The mRNA expression of IGF1 and IGF2, IGF1 and IGFR2, IGFBP2 and IGFBP4 was significantly higher (P < 0.01) in oocytes from Holstein whereas the expression of PAPP-A was significantly higher (P < 0.05) in oocytes from Nelore cows. The high PAPP-A expression and the low expression of IGFBP2 and IGFBP4 are associated with more efficient degradation of IGFBPs, which results in greater bioavailability of IGF in Nelore oocytes when compared to the Holstein. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：155 / 158
页数：4
相关论文
共 50 条
  • [21] Influence of biostimulation and temporary weaning on follicular dynamics and pregnancy rates in Nelore cows (Bos taurus indicus)
    Manoel Lopes Silva Filho
    Leilson Rocha Bezerra
    José Carlos Ferreira-Silva
    Francisco Maciel Póvoas Paulo Souto
    Ney Romulus Paula Oliveira
    Paulo Fernandes de Lima
    Claudio Coutinho Bartholomew
    Marcos Antonio Lemos de Oliveira
    Tropical Animal Health and Production, 2015, 47 : 1285 - 1291
  • [22] Blood constituents of Nelore cattle (Bos indicus), Holstein cattle (Bos taurus) and Murrah buffalo (Bubalus bubalis) cows during pregnancy, at the time of calving and at post-partum
    Fagliari, JJ
    Santana, AE
    Marchio, W
    Campos, E
    Curi, PR
    ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA, 1998, 50 (03) : 273 - 282
  • [23] INFLUENCE OF PRESELECTED HEAT TOLERANT BULLS (BOS-TAURUS-TAURUS) ON THE DEVELOPMENT OF THEIR OFFSPRING FROM PAIRING WITH NELORE-ZEBU COWS (BOS-TAURUS-INDICUS) UNDER TROPICAL CONDITIONS
    STEPHAN, E
    MERKT, H
    TIERARZTLICHE UMSCHAU, 1986, 41 (05): : 362 - &
  • [24] Ovarian function in Nelore (Bos taurus indicus) cows after post-ovulation hormonal treatments
    Machado, R.
    Bergamaschi, M. A. C. M.
    Barbosa, R. T.
    de Oliveira, C. A.
    Binelli, M.
    THERIOGENOLOGY, 2008, 69 (07) : 798 - 804
  • [25] Influence of biostimulation and temporary weaning on follicular dynamics and pregnancy rates in Nelore cows (Bos taurus indicus)
    Silva Filho, Manoel Lopes
    Bezerra, Leilson Rocha
    Ferreira-Silva, Jose Carlos
    Povoas Paulo Souto, Francisco Maciel
    Paula Oliveira, Ney Romulus
    de Lima, Paulo Fernandes
    Bartholomew, Claudio Coutinho
    Lemos de Oliveira, Marcos Antonio
    TROPICAL ANIMAL HEALTH AND PRODUCTION, 2015, 47 (07) : 1285 - 1291
  • [26] Differential expression of cytokines in PBMC of Bos indicus and Bos taurus x Bos indicus cattle due to Brucella abortus S19 antigen
    Kumar, D. Ravi
    Sivalingam, Jayakumar
    Mishra, Shailendra K.
    Kumar, Anshuman
    Vineeth, M. R.
    Chaudhuri, Pallab
    Kataria, R. S.
    Niranjan, S. K.
    ANIMAL BIOTECHNOLOGY, 2020, 31 (02) : 148 - 154
  • [27] Assessment of HSP70-1 transcription levels in immature oocytes from Bos taurus and Bos indicus cows raised in a tropical climate
    Camargo, L. S. A.
    Viana, J. H. M.
    Serapiao, R. V.
    Guimaraes, M. F. M.
    Sá, W. F.
    Ferreira, A. M.
    REPRODUCTION FERTILITY AND DEVELOPMENT, 2007, 19 (01) : 247 - 248
  • [28] Plasma anti-Mtillerian hormone as a predictive endocrine marker to select Bos taurus (Holstein) and Bos indicus (Nelore) calves for in vitro embryo production
    Batista, E. O. S.
    Guerreiro, B. M.
    Freitas, B. G.
    Silva, J. C. B.
    Vieira, L. M.
    Ferreira, R. M.
    Rezende, R. G.
    Basso, A. C.
    Lopes, R. N. V. R.
    Renno, F. P.
    Souza, A. H.
    Baruselli, P. S.
    DOMESTIC ANIMAL ENDOCRINOLOGY, 2016, 54 : 1 - 9
  • [29] Hepatic mRNA expression of enzymes associated with progesterone metabolism and its impact on ovarian and endocrine responses in Nelore (Bos indicus) and Holstein (Bos taurus) heifers with differing feed intakes
    Batista, E. O. S.
    Sala, R. V.
    Ortolan, M. D. D. V.
    Jesus, E. F.
    Del Valle, T. A.
    Renno, F. P.
    Macabelli, C. H.
    Chiaratti, M. R.
    Souza, A. H.
    Baruselli, P. S.
    THERIOGENOLOGY, 2020, 143 : 113 - 122
  • [30] Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
    Sudano, Mateus Jose
    Landim-Alvarenga, Fernanda da Cruz
    Sartori, Roberto
    Machado, Rui
    LIVESTOCK SCIENCE, 2011, 141 (2-3) : 207 - 212
12345